•   Gas Chromatography


Gas Chromatography (GC) is an instrumental technique use to analyze organic compounds. The compounds with sufficient volatility and thermal stability could be analyzed using GC. The samples that come in different matrices such as water, soil, fertilizer, etc are extracted into organic solvents, separated from interfering substances and concentrated before introducing to the instrument. Once the sample is injected into the instrument it enters to the separation column with the carrier gas. Inside the column the compound gets separated into various components. The detector measures the components that exit from the column. Different detectors will give different types of selectivity.


In our lab we use GC with various detectors to conduct analysis of volatile organic and chlorinated organic compounds;


GC – FID/ECD   - Chlorinated organic compound/volatile organic compoundanalysis         

GC-ECD/NPD    - Chlorinated organic compound analysis

GCMS                - Volatile organic compound analysis

GCMS-MS         - Volatile organic compound analysis


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  •   High Performance Liquid Chromatography

HPLC is an instrumental analytical technique to separate compounds into their components. Unlike in normal liquid chromatography where the liquid passes through the column under gravity, HPLC uses high pressure to pass the sample mixture through the column packed with a solid adsorbent material. As in other chromatographic techniques, separation of the sample into its constituents occurs according to their affinity to the mobile and stationary phase. The eluting components that come out from the column is detected by detectors. The detectors

At ITI, we use three types of HPLC in our analytical work.

HPLC- DAD   – Pesticides, food toxins, pharmaceuticals analysis

HPLC- FLD    - Pesticides, food toxins, pharmaceuticals analysis

HPLC- RID     - Pesticides, food toxins, pharmaceuticals analysis



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  •   LCMS-MS (Liquid Chromatograph- Tandem Mass Spectrometer (QTRAP)


    LCMSMS (QTRAP) is one of the recently added high end instruments to our lab. LCMS-MS is usually a high-performance liquid chromatograph combined with two mass spectrometers. Here the injected sample which gets separated into different components in the LC column is directed into the first mass spectrometer where it is ionized. The resulting ions are then separated and detected in the second mass analyzer. The results of LSMS-MS in more accurate and précised with better selectivity.

    LCMS-MS is highly used in analysis of polar organic compounds in our lab.




  •   Atomic Absorption Spectrophotometry


Atomic Absorption Spectrophotometeris a scientific instrument used to detect metals in solutions. Here the sample is fragmented into small metal atoms using anatomizer. These metal atoms interact with radiation of a pre-set wavelength which can be measured and interpreted. AAS can determine metals in parts per million to parts per billion concentration ranges with speed and affordable cost.

AAS-VGA       - Element Analysis

AAS-GTA       - Element Analysis


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  •   Inductively Coupled Plasma Mass Spectrometer


ICPMS is another latest addition to our Residual Analysis Laboratory. It is also an analytical technique used for elemental analysis. ICPMS is a type of mass spectrometer which is combined with inductively coupled Plasma. This instrument has the capability to detect metals at concentrations as low as one part in 1015




Last Updated on Thursday, 12 July 2018 11:25



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